Acid phosphatase activity in human semen

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Human prostatic acid phosphatase has phosphotyrosyl protein phosphatase activity.

The major secreted isoenzyme of human prostatic acid phosphatase (PAcP) (EC 3.1.3.2), which catalyses p-nitrophenyl phosphate (PNPP) hydrolysis at acid pH values, was found to have phosphotyrosyl protein phosphatase activity since it dephosphorylated three different phosphotyrosine-containing protein substrates. Several lines of evidence are presented to show that the phosphotyrosyl phosphatase...

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Sialic Acid in Human Semen

Sialic acid of seminal fluid of eighty two human subjects has been studied in relation to infertility. Normozoospermic group had highest sialic acid content (94 mgm/100 mt of the fluid) and was sign ificantly different from other groups. The lowest sialic acid level was observed: n necrozoosperm ic group (54 mgm/1 00 mt of the fluid). Azoospermic (62 mgm/100 ml) and vasectomised group (73 mgm/1...

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Determination of acid phosphatase activity in normal human lymphocytes.

Acid phosphatase levels in pure lymphocytes from normal individuals were determined cytochemically on intact cells and spectrophotometrically on cell extracts. Good correlation was demonstrated between the results obtained with both methods. Biochemical assay showed that a normal range of 7.2 +/- 0.9 and 7.1 +/- 1.0 mMoles per 10(6) lymphocytes was obtained without and with tartrate inhibition,...

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Original Article: Study of Semen Acid and Alkaline Phosphatase

In the present study phosphatase levels ( Acid and Alkaline ) were compared with sperm count and motility in 100 subjects with varying conditions. All the subjects presented were between 21-45 years of age. Samples of semen were collected from 100 cases comprising of the different grades of sperm concentration and motility. Human seminal fluid contains significant amount of acid phosphatase and...

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Acid Phosphatase Activity in Auer Bodies.

UER BODIES and leukocyte granules appear to be closely related structures, on the basis of similarities found in electron microscopi&’2 and histochemical stildies.3’4 One of the enzymes which can be demonstrated in leukocyte granules both by histochemical5’6’7 and biochemical methods8’#{176} is acid phosphatase. Because we considered Auer bodies to be abnormal granules, this study was undertake...

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ژورنال

عنوان ژورنال: Archives of Andrology

سال: 1979

ISSN: 0148-5016

DOI: 10.3109/01485017908985053